Preparation of human primary macrophages to study the polarization from monocyte-derived macrophages to pro- or anti-inflammatory macrophages at biomaterial interface in vitro
Background/purpose: Testing of dental materials when in contact with innate immune cells has been so far hindered L-Glutathione by the lack of proper in vitro models.Human primary monocyte-derived macrophages (MDMs) would be an excellent option to this aim.However, the inability to detach them from the tissue culture plates contrast the possibility